Project

Publication Information (Click the authors' names to retrieve the abstract)

Flow Cytometry/Population Modeling

Gilbert A, Srienc F.

Optimized evolution in the cytostat: a Monte Carlo simulation. Biotechnol Bioeng [Epub ahead of print]

Flow Cytometry

Sitton G, Srienc F.

Mammalian cell culture scale-up and fed-batch control using automated flow cytometry. J Biotechnol. 2008 Jun 1;135(2):174-80.

Flow Cytometry

Sitton G, Srienc F.

Growth dynamics of mammalian cells monitored with automated cell cycle staining and flow cytometry. Cytometry A. 2008 Jun;73(6):538-45.

Metabolic Modeling/Engineering

Trinh CT, Unrean P, Srienc F.

A Minimal Escherichia coli Cell for most Efficient Ethanol Production from Hexoses and Pentoses. Appl Environ Microbiol. 2008 Jun;74(12):3634-43.

Gorke JT, Srienc F, Kazlauskas RJ.

Hydrolase-catalyzed biotransformations in deep eutectic solvents. Chem Commun (Camb). 2008 Mar 14;(10):1235-7.

Polyhydroxyalkanoate Production

McChalicher CW, Srienc F.

Investigating the structure-property relationship of bacterial PHA block copolymers. J Biotechnol. 2007 Nov 1;132(3):296-302.

Polyhydroxyalkanoate Production

Gorke JT, Okrasa K, Louwagie A, Kazlauskas RJ, Srienc F.

Enzymatic synthesis of poly(hydroxyalkanoates) in ionic liquids. J Biotechnol. 2007 Nov 1;132(3):306-13.

Flow Cytometry

Sitton, G, Hansgate, A, Srienc, F.

Transient gene expression in CHO cells monitored with automated flow cytometry. Cytotechnology. 2006 Sept.

Metabolic Modeling/Engineering

Trinh, C.T., Carlson, R., Wlaschin, A. and Srienc, F.

Design, construction and performance of the most efficient biomass producing E. coli bacterium. 2006 Nov;8(6):628-38.

Flow Cytometry

Kacmar, J., Gilbert, A., Cockrell, J., Srienc, F.

The cytostat: A new way to study cell physiology in a precisely defined environment
J Biotechnol. 2006 Nov 1;126(2):163-72.

Polyhydroxyalkanoate Production

Pederson, EN., McChalicher, CWJ., Srienc, F.

Bacterial Synthesis of PHA Block Copolymers
Biomacromolecules, 2006 Jun;7(6):1904-11.

Systems Biology

Sangurdekar DP, Srienc F, Khodursky AB.

A classification based framework for quantitative description of large-scale microarray data.
Genome Biol. 2006;7(4):R32. Epub 2006 Apr 20.

Polyhydroxyalkanoate production, Metabolic Modeling/Engineering

Wlaschin AP, Trinh CT, Carlson R, Srienc F.

The fractional contributions of elementary modes to the metabolism of Escherichia coli and their estimation from reaction entropies.
Metab Eng. 2006 Jul;8(4):338-52.

Polyhydroxyalkanoate production

Carlson, R., and Srienc, F.

Effects of recombinant precursor pathway variations on poly[(R)-3-hydroxybutyrate] synthesis in Saccharomyces cerevisiae.
2006 Jul 25;124(3):561-73. Epub 2006 Mar 10.

Polyhydroxyalkanoate production

Zhang, B., Carlson, R., and Srienc, F.

Engineering the Monomer Composition of Polyhydroxyalkanoates Synthesized in Saccharomyces cerevisiae.
Appl Environ Microbiol. 2006 Jan;72(1):536-43.

Flow cytometry, Polyhydroxyalkanoate production

Kacmar J, Carlson R, Balogh SJ, Srienc F.

Staining and quantification of poly-3-hydroxybutyrate in Saccharomyces cerevisiae and Cupriavidus necator cell populations using automated flow cytometry.
Cytometry A. 2006 Jan;69(1):27-35.

Flow cytometry

Kacmar J, Srienc F.

Dynamics of single cell property distributions in Chinese hamster ovary cell cultures monitored and controlled with automated flow cytometry.
J Biotechnol. 2005 Dec 6;120(4):410-420.

Polyhydroxyalkanoate production

Vijayasankaran N, Carlson R, Srienc F.

Synthesis of Poly[(R)-3-hydroxybutyric acid) in the Cytoplasm of Pichia pastoris under Oxygen Limitation.
Biomacromolecules. 2005 Mar 14;6(2):604-611.

Metabolic modeling

Vijayasankaran N, Carlson R, Srienc F.

Metabolic pathway structures for recombinant protein synthesis in Escherichia coli.
Appl Microbiol Biotechnol. 2005 Mar 1

Polyhydroxyalkanoate production

Carlson R, Wlaschin A, Srienc F.

Kinetic studies and biochemical pathway analysis of anaerobic poly-(R)-3-hydroxybutyric acid synthesis in Escherichia coli.
Appl Environ Microbiol. 2005 Feb;71(2):713-20.

Polyhydroxyalkanoate production

Pederson EN, Srienc F.

Mass spectrometry feedback control for synthesis of polyhydroxyalkanoate granule microstructures in Ralstonia eutropha.
Macromol Biosci. 2004 Mar 15;4(3):243-54.

Flow cytometry

Kacmar J, Zamamiri A, Carlson R, Abu-Absi NR, Srienc F.

Single-cell variability in growing Saccharomyces cerevisiae cell populations measured with automated flow cytometry.
J Biotechnol. 2004 Apr 29;109(3):253-68.

Lotti M, Porro D, Srienc F.

Recombinant proteins and host cell physiology.
J Biotechnol. 2004 Apr 8;109(1-2):1-2.

Metabolic modeling

Carlson R, Srienc F.

Fundamental Escherichia coli biochemical pathways for biomass and energy production: creation of overall flux states.
Biotechnol Bioeng. 2004 Apr 20;86(2):149-62.

Metabolic modeling

Carlson R, Srienc F.

Fundamental Escherichia coli biochemical pathways for biomass and energy production: identification of reactions.
Biotechnol Bioeng. 2004 Jan 5;85(1):1-19.

Flow cytometry

Abu-Absi NR, Zamamiri A, Kacmar J, Balogh SJ, Srienc F.

Automated flow cytometry for acquisition of time-dependent population data.
Cytometry. 2003 Feb;51A(2):87-96.

Metabolic modeling

Carlson R, Fell D, Srienc F.

Metabolic pathway analysis of a recombinant yeast for rational strain development.
Biotechnol Bioeng. 2002 Jul 20;79(2):121-34.

Flow cytometry

Abu-Absi NR, Srienc F.

Instantaneous evaluation of mammalian cell culture growth rates through analysis of the mitotic index.
J Biotechnol. 2002 Apr 25;95(1):63-84.

Flow cytometry

Patkar A, Vijayasankaran N, Urry DW, Srienc F.

Flow cytometry as a useful tool for process development: rapid evaluation of expression systems.
J Biotechnol. 2002 Feb 28;93(3):217-29.

Flow cytometry

Frykman S, Srienc F.

Cell cycle-dependent protein secretion by Saccharomyces cerevisiae.
Biotechnol Bioeng. 2001 Nov;76(3):259-68.

Population modeling

Mantzaris,N.V., Daoutidis,P., & Srienc,F.

Numerical Solution of Multivariable Cell Population Balance Models: III. Finite Element Methods. Comp.& Chem.Eng. Volume 25, Issues 11-12, 15 November 2001, Pages 1463-1481.

Population modeling

Mantzaris,N.V., Daoutidis,P., & Srienc,F.

Numerical Solution of Multivariable Cell Population Balance Models: II. Spectral Methods. Comp.& Chem.Eng. Volume 25, Issues 11-12, 15 November 2001, Pages 1441-1462.

Population modeling

Mantzaris,N.V., Daoutidis,P., & Srienc,F.

Numerical Solution of Multivariable Cell Population Balance Models: I. Finite Difference Methods. Comp.& Chem.Eng. Volume 25, Issues 11-12, 15 November 2001, Pages 1411-1440.

Population modeling, Polyhydroxyalkanoate production

Mantzaris,N.V., Kelley,A.S., Daoutidis,P., & Srienc,F (2001) Controlled Synthesis of Polyhydroxyalkanoic (PHA) Nanostructures in R. eutropha Kelley, A. S.; Mantzaris, N. V.; Daoutidis, P.; Srienc, F.; Nano Lett.; (Communication); 2001; 1(9); 481-485.

Population modeling, Polyhydroxyalkanoate production

Mantzaris,N.V., Kelley,A.S., Daoutidis,P., & Srienc,F (2001)

The Design of an Optimal Carbon Source Switching Strategy for the Production of PHBV-PHB and PHB-PHBV di-Block Copolymers in a Ralstonia Eutropha Cell Population. AIChE J. 47, 727-743.

Population modeling

Mantzaris,N.V., Daoutidis,P., & Srienc,F.

Nonlinear Productivity Control using a Multi-Staged Cell Cell Population Balance Model. Chem.Eng.Sci. Volume 57, Issue 1, January 2002, Pages 1-14

Flow cytometry

Bratvold,D., Srienc,F., & Taub,S.R. (2000)

Analysis of the distribution of ingested bacteria in nanoflagellates and estimation of grazing rates with flow cytomery. Aquatic Microbial Ecology 21, 1-12.

Polyhydroxyalkanoate production

Srienc,F. & Carlson,R. (2000)

Metabolic Engineering of Polyhydroxyalkanoate Synthesis in Eukaryotic Cells. Proc.2000 Agric.Biotechnol.Symp. 90-103.

Flow cytometry

Natarajan A, Srienc F.

Glucose uptake rates of single E. coli cells grown in glucose-limited chemostat cultures.
J Microbiol Methods. 2000 Sep;42(1):87-96.

Flow cytometry

Alberghina L, Porro D, Shapiro H, Srienc F, Steen H.

Microbial analysis at the single-cell level
J Microbiol Methods. 2000 Sep;42(1):1-2.

Natarajan A, Everard M, Manias D, Srienc F, Dunny G.

A sensitive method to detect initiation of growth in Streptococcus gordonii using ribosomal RNA operon-reporter gene fusions.
J Microbiol Methods. 2000 Jan;39(2):133-43.